Biosynthesis, partial purification and characterization of invertase through Carrot (Daucus carota L.) Peels

The current work was investigated on the production of Invertase under solidstate fermentation (SSF) through Aspergillus species, by utilizing peels of Carrot (Daucus carota L.) as a substrate. The maximum production of Invertase (7.95±0.1 U/ml) was attained by using Aspergillus niger with 1×106 spores/ml at 90% moisture content after incubation period of 72 h. Through ammonium sulphate precipitation the enzyme was purified about 1.42-fold. Invertase showed maximum thermal stability ranges from 20-70°C at a pH range (5.5 to 6.5) with utmost activity at pH 5.5 and 50°C. However, the enzyme was highly active at two different concentrations of sucrose that is, 0.1 and 0.5 M respectively, but it indicates less activity at glycerol. While the enzyme was completely repressed by Hg2+ (1 mM) and slightly enthused by Co2+ and Na+ at the similar application.